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nk 92 cell specific culture medium  (Procell Inc)

 
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    Structured Review

    Procell Inc nk 92 cell specific culture medium
    Characteristics of isolated exosomes and internalization <t>by</t> <t>NK-92</t> cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.
    Nk 92 Cell Specific Culture Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nk 92 cell specific culture medium/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    nk 92 cell specific culture medium - by Bioz Stars, 2026-06
    86/100 stars

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    1) Product Images from "Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells"

    Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-36706-9

    Characteristics of isolated exosomes and internalization by NK-92 cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.
    Figure Legend Snippet: Characteristics of isolated exosomes and internalization by NK-92 cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.

    Techniques Used: Isolation, Western Blot, Fluorescence

    Apoptosis of NK-92 cells induced by CCA-Exos. ( A ) Flow cytometry assays showing apoptosis of NK-92 cells induced by RBE-Exos. ( B ) Comparison of apoptosis rate of NK-92 cells induced by RBE-Exos and PBS. ( C ) Flow cytometry assays showing apoptosis of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. ( D ) Comparison of apoptosis rate of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( E ) Protein levels of BCL-2 in NK-92 cells treated with RBE-Exos or PBS. ( F ) Protein levels of BCL-XL in NK-92 cells treated with RBE-Exos or PBS. ( G ) Protein levels of BAX in NK-92 cells treated with RBE-Exos or PBS. ( H ) Protein levels of BAD in NK-92 cells treated with RBE-Exos or PBS. ( I ) Relative transcriptional levels of BCL-2 , BCL-XL , BAX , and BAD of NK-92 cells treated with RBE-Exos or PBS. Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.
    Figure Legend Snippet: Apoptosis of NK-92 cells induced by CCA-Exos. ( A ) Flow cytometry assays showing apoptosis of NK-92 cells induced by RBE-Exos. ( B ) Comparison of apoptosis rate of NK-92 cells induced by RBE-Exos and PBS. ( C ) Flow cytometry assays showing apoptosis of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. ( D ) Comparison of apoptosis rate of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( E ) Protein levels of BCL-2 in NK-92 cells treated with RBE-Exos or PBS. ( F ) Protein levels of BCL-XL in NK-92 cells treated with RBE-Exos or PBS. ( G ) Protein levels of BAX in NK-92 cells treated with RBE-Exos or PBS. ( H ) Protein levels of BAD in NK-92 cells treated with RBE-Exos or PBS. ( I ) Relative transcriptional levels of BCL-2 , BCL-XL , BAX , and BAD of NK-92 cells treated with RBE-Exos or PBS. Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Techniques Used: Flow Cytometry, Comparison

    Proliferation, production of effectors and killing activity of NK-92 cells. ( A ) Proliferation of NK-92 cells treated with RBE-Exos or PBS. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with RBE-Exos or PBS. ( B ) Proliferation of NK-92 cells treated with serum exosomes. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with CCA serum-Exos or healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( C ) Production of effectors. ELISA showed that RBE-Exos decreased the production of IFN-γ and granzyme B obviously but not TNF-α and perforin. ( D ) Cytotoxicity of NK-92 cells treated with RBE-Exos or PBS. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by RBE-Exos. ( E ) Cytotoxicity of NK-92 cells treated serum exosomes. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by CCA serum-Exos. Data were presented as the mean ± SEM from n = 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
    Figure Legend Snippet: Proliferation, production of effectors and killing activity of NK-92 cells. ( A ) Proliferation of NK-92 cells treated with RBE-Exos or PBS. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with RBE-Exos or PBS. ( B ) Proliferation of NK-92 cells treated with serum exosomes. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with CCA serum-Exos or healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( C ) Production of effectors. ELISA showed that RBE-Exos decreased the production of IFN-γ and granzyme B obviously but not TNF-α and perforin. ( D ) Cytotoxicity of NK-92 cells treated with RBE-Exos or PBS. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by RBE-Exos. ( E ) Cytotoxicity of NK-92 cells treated serum exosomes. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by CCA serum-Exos. Data were presented as the mean ± SEM from n = 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Techniques Used: Activity Assay, Enzyme-linked Immunosorbent Assay

    Effect of RBE-Exos on the adhesion of NK-92 cells. ( A ) Growth status of NK-92 cells. NK cells treated with RBE-Exos lost the normal cluster growing. ( B ) CD11a protein levels. ( C ) CD18 protein levels. ( D ) CD54 protein levels. Western blot showed that the protein levels of CD11a, CD18 and, CD54 were obviously decreased in NK-92 cells treated with RBE-Exos. ( E ) Relative transcriptional levels of CD11a , CD18 , CD54 and, CD2 in NK-92 cells. qRT-PCR displayed that RBE-Exos downregulated the transcriptional levels of CD11a , CD18 , CD54 , but not CD2 . Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.
    Figure Legend Snippet: Effect of RBE-Exos on the adhesion of NK-92 cells. ( A ) Growth status of NK-92 cells. NK cells treated with RBE-Exos lost the normal cluster growing. ( B ) CD11a protein levels. ( C ) CD18 protein levels. ( D ) CD54 protein levels. Western blot showed that the protein levels of CD11a, CD18 and, CD54 were obviously decreased in NK-92 cells treated with RBE-Exos. ( E ) Relative transcriptional levels of CD11a , CD18 , CD54 and, CD2 in NK-92 cells. qRT-PCR displayed that RBE-Exos downregulated the transcriptional levels of CD11a , CD18 , CD54 , but not CD2 . Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Techniques Used: Western Blot, Quantitative RT-PCR



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    nk 92 cell specific culture medium  (Procell Inc)
    86
    Procell Inc nk 92 cell specific culture medium
    Characteristics of isolated exosomes and internalization <t>by</t> <t>NK-92</t> cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.
    Nk 92 Cell Specific Culture Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nk 92 cell specific culture medium/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    nk 92 cell specific culture medium - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Characteristics of isolated exosomes and internalization by NK-92 cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.

    Journal: Scientific Reports

    Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

    doi: 10.1038/s41598-026-36706-9

    Figure Lengend Snippet: Characteristics of isolated exosomes and internalization by NK-92 cells. ( A ) Morphologies of exosomes isolated from RBE cells, CCA patient serum and healthy people serum respectively under TEM. Yellow arrows indicate the exosomes. ( B ) NTA of exosomes. ( C ) Identification of exosomes by western blot analysis for HSP70 and CD63. ( D ) Tracing and internalization of CCA-Exos by NK-92 cells. Green, blue, and red fluorescence represent exosomes, nucleus and cytoskeleton of NK-92 cells. The three figures were merged into the fourth showing exosomes entered the cells.

    Article Snippet: NK-92 cells were cultured in NK-92 cell specific culture medium (Procell, Wuhan, China) with 200 U/mL IL-2 (Procell, Wuhan, China).

    Techniques: Isolation, Western Blot, Fluorescence

    Apoptosis of NK-92 cells induced by CCA-Exos. ( A ) Flow cytometry assays showing apoptosis of NK-92 cells induced by RBE-Exos. ( B ) Comparison of apoptosis rate of NK-92 cells induced by RBE-Exos and PBS. ( C ) Flow cytometry assays showing apoptosis of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. ( D ) Comparison of apoptosis rate of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( E ) Protein levels of BCL-2 in NK-92 cells treated with RBE-Exos or PBS. ( F ) Protein levels of BCL-XL in NK-92 cells treated with RBE-Exos or PBS. ( G ) Protein levels of BAX in NK-92 cells treated with RBE-Exos or PBS. ( H ) Protein levels of BAD in NK-92 cells treated with RBE-Exos or PBS. ( I ) Relative transcriptional levels of BCL-2 , BCL-XL , BAX , and BAD of NK-92 cells treated with RBE-Exos or PBS. Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Scientific Reports

    Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

    doi: 10.1038/s41598-026-36706-9

    Figure Lengend Snippet: Apoptosis of NK-92 cells induced by CCA-Exos. ( A ) Flow cytometry assays showing apoptosis of NK-92 cells induced by RBE-Exos. ( B ) Comparison of apoptosis rate of NK-92 cells induced by RBE-Exos and PBS. ( C ) Flow cytometry assays showing apoptosis of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. ( D ) Comparison of apoptosis rate of NK-92 cells induced by CCA serum-Exos and healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( E ) Protein levels of BCL-2 in NK-92 cells treated with RBE-Exos or PBS. ( F ) Protein levels of BCL-XL in NK-92 cells treated with RBE-Exos or PBS. ( G ) Protein levels of BAX in NK-92 cells treated with RBE-Exos or PBS. ( H ) Protein levels of BAD in NK-92 cells treated with RBE-Exos or PBS. ( I ) Relative transcriptional levels of BCL-2 , BCL-XL , BAX , and BAD of NK-92 cells treated with RBE-Exos or PBS. Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: NK-92 cells were cultured in NK-92 cell specific culture medium (Procell, Wuhan, China) with 200 U/mL IL-2 (Procell, Wuhan, China).

    Techniques: Flow Cytometry, Comparison

    Proliferation, production of effectors and killing activity of NK-92 cells. ( A ) Proliferation of NK-92 cells treated with RBE-Exos or PBS. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with RBE-Exos or PBS. ( B ) Proliferation of NK-92 cells treated with serum exosomes. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with CCA serum-Exos or healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( C ) Production of effectors. ELISA showed that RBE-Exos decreased the production of IFN-γ and granzyme B obviously but not TNF-α and perforin. ( D ) Cytotoxicity of NK-92 cells treated with RBE-Exos or PBS. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by RBE-Exos. ( E ) Cytotoxicity of NK-92 cells treated serum exosomes. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by CCA serum-Exos. Data were presented as the mean ± SEM from n = 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: Scientific Reports

    Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

    doi: 10.1038/s41598-026-36706-9

    Figure Lengend Snippet: Proliferation, production of effectors and killing activity of NK-92 cells. ( A ) Proliferation of NK-92 cells treated with RBE-Exos or PBS. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with RBE-Exos or PBS. ( B ) Proliferation of NK-92 cells treated with serum exosomes. CCK8 detecting showed no significance between proliferation of NK-92 cells treated with CCA serum-Exos or healthy serum-Exos. Data are presented as the mean ± SEM from n = 3 independent experiments. ( C ) Production of effectors. ELISA showed that RBE-Exos decreased the production of IFN-γ and granzyme B obviously but not TNF-α and perforin. ( D ) Cytotoxicity of NK-92 cells treated with RBE-Exos or PBS. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by RBE-Exos. ( E ) Cytotoxicity of NK-92 cells treated serum exosomes. LDH detection displayed the cytotoxicity of NK-92 cells was suppressed by CCA serum-Exos. Data were presented as the mean ± SEM from n = 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: NK-92 cells were cultured in NK-92 cell specific culture medium (Procell, Wuhan, China) with 200 U/mL IL-2 (Procell, Wuhan, China).

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay

    Effect of RBE-Exos on the adhesion of NK-92 cells. ( A ) Growth status of NK-92 cells. NK cells treated with RBE-Exos lost the normal cluster growing. ( B ) CD11a protein levels. ( C ) CD18 protein levels. ( D ) CD54 protein levels. Western blot showed that the protein levels of CD11a, CD18 and, CD54 were obviously decreased in NK-92 cells treated with RBE-Exos. ( E ) Relative transcriptional levels of CD11a , CD18 , CD54 and, CD2 in NK-92 cells. qRT-PCR displayed that RBE-Exos downregulated the transcriptional levels of CD11a , CD18 , CD54 , but not CD2 . Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Scientific Reports

    Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

    doi: 10.1038/s41598-026-36706-9

    Figure Lengend Snippet: Effect of RBE-Exos on the adhesion of NK-92 cells. ( A ) Growth status of NK-92 cells. NK cells treated with RBE-Exos lost the normal cluster growing. ( B ) CD11a protein levels. ( C ) CD18 protein levels. ( D ) CD54 protein levels. Western blot showed that the protein levels of CD11a, CD18 and, CD54 were obviously decreased in NK-92 cells treated with RBE-Exos. ( E ) Relative transcriptional levels of CD11a , CD18 , CD54 and, CD2 in NK-92 cells. qRT-PCR displayed that RBE-Exos downregulated the transcriptional levels of CD11a , CD18 , CD54 , but not CD2 . Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: NK-92 cells were cultured in NK-92 cell specific culture medium (Procell, Wuhan, China) with 200 U/mL IL-2 (Procell, Wuhan, China).

    Techniques: Western Blot, Quantitative RT-PCR